It is one of the most used tests for determining the antioxidant activity of plant extracts. Study of total phenolic and flavonoid content, antioxidant activity and antimicrobial properties of medicinal plants volume 1 issue 1 2014 sarabjot kaur,1 poonam mondal2 1department of pharmaceutical sciences, assam university, india 2department of. Quantification of the antioxidant activity of plant. Evaluation of antioxidant activity of two important memory. The extract of medicinal plants were prepared using soxhlet apparatus for alcoholic extract, and water reflux for. Hatbased methods measure the classical ability of an antioxidant to quench free radicals by hydrogen donation ah any h donor hence, many scientists feel these are most relevant to reactions where antioxidants typically act. This thesis deals with screening of antioxidant activity, phenols content and mineral profiles, optimisation of extraction of antioxidants and optimisation of spray drying of boiling extract from the selected lamiaceae plants. Antioxidant activity of herbaceous plant extracts protect. The study showed that the plant revealed an antioxidant activity 80% with ic 50 0. Herbaceous plants containing antioxidants can protect against dna damage. View phytochemical screening, antimicrobial and antioxidant activity of plants.
Implications for alzheimers disease therapy by salam yousef jamil abu zaitoun supervisor dr. I warmly thank to commitee members of my thesis, assist. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. There is an increasing interest in natural antioxidants e. Quantification of bioactive compounds and evaluation of.
These results confirm the ethnobotanical claims by traditional medical practitioners treating viral, bacterial and fungal infections caused by hivaids, cancer and cardiovascular diseases with traditional medicinal plants due to the rich phytochemistry, their high levels of antioxidant activity as well as bioactivity of the plants. Both ethanol and acetone extracts of these latexes showed good scavenging activity. The antioxidative activity of a total of 92 phenolic extracts from edible and nonedible plant materials berries, fruits, vegetables, herbs, cereals, tree materials, plant sprouts, and seeds was examined by autoxidation of methyl linoleate. Plants are an important source of natural active products that are different, based on mechanism and biological properties. Phenolic compounds such as flavonoids are ubiquitous within the plant kingdom. Comparative analysis of antioxidant activity in various. Invitro evaluation of acetylcholinesterase inhibition and antioxidant activity of selected palestinian medicinal plants. The emergence and spread of antibiotic resistance, as well as the evolution of new strains of disease causing agents, are of great concern to the global health community.
Antioxidant activity of plant extracts containing phenolic. Antibacterial activity in vitro of medicinal plants. The antioxidant activity of a crude extract against dpph oxidant is described in chapter 2. The objectives of the studies presented in this thesis were to identify and quantify the major phenolic components of red wine and to assess the contribution of individual compounds to the total antioxidant activity. Methanol and ethanol have been extensively used to extract antioxidant compounds from various plants and plantbased foods fruits, vegetables etc. So the aim of the present study is to evaluate the dpph.
Over the years, research on antioxidants and medicinal plants has gained enormous popularity and emerged as a potential therapeutic to prevent free radical generated damage in the human body. Ferhat celep for his guidance in identification of plants and to dr. Joao batista texeira da rocha a thesis submitted to, ppgbqccneufsm in partial fulfillment of the requirements for the degree of doctor of philosophy phd in biochemical toxicology. Introduction biological screening procedures are crucial and necessary in order to estimate the harmful or therapeutic potential of useful drugs. The extracts were investigated for the antioxidant activity using 2, 2 diphenyl, 1 picryl hydrazyl. Phytochemical investigation and in vitro antioxidant activity of an. It has been mentioned that antioxidant activity of plants might be due to their phenolic compounds cook and samman, 1996. Minimal inhibitory concentration of metanoic extracts ranged from 6. Standardized methods for the determination of antioxidant. Studies for the determination of the antioxidant activity of different plant species could contribute to revealing the value of these species as a source of new antioxidant compounds.
The antibacterial effect of some medicinal plant extracts. Although a number of plants traditionally been used by the people of purulia district for the treatment of different diseases e. We offer assays to measure the activity of specific antioxidants. Study of total phenolic and flavonoid content, antioxidant. Thepurpose of this study was to evaluate the antioxidant substances,antioxidant activity, and protection of dna from oxidative damage in humanlymphocytes induced by hydrogen peroxide h2o2. Biological and chemical studies of medicinal plants a. Celery apium graveolens l is a plant from the apiaceae family and phenolic and antioxidant compounds of this plant have been studied by several scientists. Biological and chemical studies of medicinal plants. Screening of in vitro antioxidant activity of methanolic. Extraction and determination of antioxidant activity of. Antioxidant properties of extracts from selected plant materials caesalpinia spinosa, perilla frutescens, artemisia annua and viola wittrockiana.
Preliminary phytochemical screening of extract the methanolic extract was tested by applying general chemical tests 12 for alkaloids, glycosides, reducing sugars, tannins, fixed oils and fats, proteins and free amino acids. The effect of various plant extracts was measured on several important oxidation indicators. Antioxidant activity was evaluated by dpph radical scavenging assay. Red wines were analysed for their phenolic content and antioxidant activity using a range of complementary techniques including hplctandem mass spectrometry, preparative hplc and. Ourmethods used acidic methanol and water extractions from six herbaceousplants, including bidens alba ba, lycium. Chemistry and biological activities of medicinal plants from mali b. The objective of this thesis was to select the plant materials of the selected lamiaceae species that gave the highest antioxidant activity, highest phenols content and. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. Antioxidant activity, protective effects and absorption of. Antioxidant is a molecule that inhibits the oxi dation of other molecules. These properties are due to many active phytochemicals including. Pdf thesis antioxidant activity of amaranthus gangeticus. Cultivar differences in physicochemical properties and antioxidants were found among. In the present paper we have investigated antioxidant activity of extracts from withania somnifera dunal.
The results showed that the ethanol extracts of plant samples displayed ability to inhibit dpph free radical formation by 82%. View antioxidant activity research papers on academia. Pdf methods for determining the antioxidant activity. The most practical way to fight degenerative diseases is to increase antioxidant activity in our body and that could be achieved by consumption of vegetables, fruits or edible plants. The antioxidant activity of the aerial part extract of m. Isolation and characterisation of medicinal compounds from 19 dec 20 isolation and characterisation of medicinal phd thesis. Additionally, you may measure the antioxidant capacity of certain. Oxidation is a chemi cal reaction that transfers electron or hydrogen. The antioxidant activity of the extracts determined using the frap assay are shown in fig 2. Jay kambli 1, ashwini patil, chithrashree 2, and rohini keshava 3 1department of biotechnology, acharyas bangalore bschool, bangalore, karnataka, india, 560 091, 2biocontrol research laboratory, department of studies in microbiology and biotechnology. Effect of extraction solventtechnique on the antioxidant activity of selected medicinal plant extracts. However, due to having a large variety of medicinal plants species, all of the major medicinal plants have not yet been evaluated for their medicinal properties, including antioxidant activity. All eight crude plant extracts showed marked antioxidant activity in both assays.
Cellular antioxidant enzymes and other redox molecules serve to counterbalance ros generated in the cell. Munqez shtaya this thesis is submitted in partial fulfillment of the. Phytochemicals screening and evaluation of antioxidants. There is a large variety of in vitro methods to quantify antioxidant activity, and it is important to select the proper method to. Among the plants in this study, pimpinella anisum showed the lowest tpc value 103. Chinese medicinal plants contain a wide variety of natural antioxidants, such as phenolic acids, flavonoids and tannins and possess more potent antioxidant activity than common dietary plants cai et al. A significant correlation was established between the orac and the tbars tests, confirming that the antioxidant. Four replicates of 40 mg of deg was added to 2 ml of methanol and shaken vigorously, 1 ml of 0. I hereby declare that the dissertationthesis submitted for m tech. The content of total phenolics in the extracts was determined spectrometrically according to the folin. The bark extract of the plant showed scavenging activity of hydroxyl radicals and the extract contained polyphenols. Keeping the above in view the present chapter focuses on the i diversity of antioxidant phytochemicals in medicinal.
The results obtained presented in figure 5b show that the percentage of inhibition of the various extracts increases significantly in a dosedependent manner. Rhizophora mangle is a plant from rhizophoraceae family. A thesis submitted in partial fulfillment of the requirements for the degree of master in biological science microbiology. Libyan capparis spinose has good antioxidant activity. Assessment of antioxidant activity of plant extracts by. Antioxidant, antibacterial and antitumor activity of. Antioxidant activity in plant oil plant oils contain unsaturated fatty acids, which are most sensitive to oxidative degradation. Effect of extraction solventtechnique on the antioxidant. Dpph free radical scavenging activity of the extracts of. Recently there has been an upsurge of interest in the therapeutic potential of medicinal plants as antioxidants in reducing such free radicals, induced injury pourmorad etal. Invitro evaluation of acetylcholinesterase inhibition and.
Screening and optimisation study on antioxidant extracts. Pdf final accepted version with authors formatting. Antioxidant and hepatoprotective activity of brazilian plants, containing phenolic compounds by syed mubasher sabir supervisor prof. Antioxidant and antibacterial activities of ethanol. Analysis of selected medicinal plants as antioxidants with. The potential antioxidant activity of the extract was assessed on the basis of the scavenging activity of the 1,1diphenyl2picrylhydrazyl dpph free radical. Antibacterial and antioxidant activity of traditional.
Commonly used medicinal plants of our community could be an excellent source of drugs to fight off this. Phytochemical investigation and in vitro antioxidant. A thesis submitted in partial fulfillment of the requirements for the. A comparative study of antioxidant and physicochemical properties of blackberry and kiwifruit except where reference is made to the work of others, the work described in this thesis is my own or was done in collaboration with my advisory committee. Effective treatment of a disease entails the development of new pharmaceuticals or some potential source of novel drugs. Phytochemical investigation and in vitro antioxidant activity of an indigenous medicinal plant alpinia nigra b. Excess ros must be promptly eliminated from the cell by a variety of antioxidant defense mechanisms. This thesis does not include proprietary or classified information. In vitro antimicrobial activity of some medicinal plants. Antioxidant and antibacterial activities of ethanol extract and. Antibacterial and antioxidant activities of 8 aromatic plants from the balkan peninsula were studied. Activity of both plants was evaluated and it was observed that significantly higher p antioxidant and cytotoxic activity of ficus racemosa linn.